![]() Honda H, Hirai A (1990) A simple and efficient method for identification of hybrids using nonradiactive rDNA as probe. Hinnishaels S, Bariller L, Mouras A, Sidorov V, Del-Favero J, Veuskens J, Negrutu I (1991) Highly asymmetric intergeneric nuclear hybrids between Nicotiana and Petunia: evidence for recombinogenic and translocation events in somatic hybrid plants after ‘gamma”-fusion. Gupta PP, Schieder O, Gupta M (1984) Intergeneric nuclear gene transfer between somatically and sexually incompatible plants through asymmetric protoplast fusion. Gupta PP, Gupta M, Schieder O (1982) Correction of nitrate reductase defect in auxotrophic plant cells through protoplast-mediated intergeneric gene transfer. Gleba YY, Hinnisdaels S, Sidorov VA, Kaleda VA, Parokonny AS, Boryshuk NV, Cherep NN, Negrutiu I, Jacobs M (1988) Intergeneric asymmetric hybrids between Nicotiana plumbaginifolia and Atropa belladonna obtained by “gamma-fusion”. Proc Natl Acad Sci USA 80:8434–8438įeher A, Preiszner Z, Litkey Z, Csanadi G, Dudits D (1992) Characterization of chromosome instability interspecific somatic hybrids obtained by X-ray fusion between potato ( Solanum tuberosum L.) and S. Mol Gen Genet 179:283–288ĭudits D, Maroy E, Praznovszky T, Olah Z, Gyorgyey J, Cella R (1987) Transfer of resistance traits from carrot into tobaco by asymmetric somatic hybridization: regeneration of fertile plants. Theor Appl Genet 62:301–304ĭudits D, Fejer O, Hadlaczky G, Lazar GB, Horvath G (1980) Intergeneric gene transfer mediated by plants protoplast fusion. Theor Appl Genet 74:718–726Ĭhien YC, Kao KN, Wetter LR (1982) Chromosome and isozyme studies of Nicotiana tabacum- Glycine max. However 1 of the regenerated plants had a novel band pattern of mtDNA that was not detected in either of the parents, indicating a possible recombination of mitochondrial genomes.īates GW, Hasenkampf CA, Contolini CL, Piastuch WC (1987) Asymmetric hybridization in Nicotiana by fusion of irradiated protoplasts. CpDNA patterns of the regenerated plants were indistinguishable from those of the carrot parent. Chloroplast (cp) and mitochondrial (mt) DNAs were also analyzed by Southern hybridization by using several probes. A Southern hybridization analysis with non-radioactively labelled DNA fragments of the rgp1 gene showed that regenerated plants had hybridizing bands from both rice and carrot. ![]() An isozyme analysis revealed that several regenerated plants had the peroxidase isozyme patterns of both parents. A cytological analysis of callus cultures induced from these plants indicated that most of the cells possessed 20–22 chromosomes and were resistant to 5MT. The morphologies of most of the regenerated plants closely resembled that of the parental carrot plants. Somatic hybrids were regenerated from 5 of the 5MT-resistant colonies. The complementary recovered cells divided and formed colonies, which were then cultivated on regeneration medium supplemented with 25mg/l 5MT to eliminate any escaped carrot cells. X-ray-irradiated protoplasts isolated from a cytoplasmic malesterile (cms) carrot suspension culture were fused with iodoacetoamide-treated protoplasts isolated from a 5-methyltryptophan (5MT)-resistant rice suspension culture by electrofusion. Asymmetric hybrid plants were obtained from fused protoplasts of a monocotyledon ( Oryza sativa L.) and a dicotyledon ( Daucus carota L.).
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